7q2q | pdb_00007q2q
cryo iDPC-STEM structure recorded with CSA 3.5
Structural highlights
FunctionCAPSD_TMV Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA. Publication Abstract from PubMedIn electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC-STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 A resolution, as well as for TMV at 3.5 A resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC-STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules. Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.,Lazic I, Wirix M, Leidl ML, de Haas F, Mann D, Beckers M, Pechnikova EV, Muller-Caspary K, Egoavil R, Bosch EGT, Sachse C Nat Methods. 2022 Sep;19(9):1126-1136. doi: 10.1038/s41592-022-01586-0. Epub 2022 , Sep 5. PMID:36064775[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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