7q5n

Crystal structure of Chaetomium thermophilum Ahp1-Urm1 complex

spinqualitylabelsall models 7q5n, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7q5n is a 12 chain structure with sequence from Chaetomium thermophilum var. thermophilum DSM 1495. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: CSS, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G0S1P8_CHATD Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.[RuleBase:RU366011] Publication Abstract from PubMed Post-translational modifications by ubiquitin-like proteins (UBLs) are essential for nearly all cellular processes. Ubiquitin-related modifier 1 (Urm1) is a unique UBL, which plays a key role in tRNA anticodon thiolation as a sulfur carrier protein (SCP) and is linked to the noncanonical E1 enzyme Uba4 (ubiquitin-like protein activator 4). While Urm1 has also been observed to conjugate to target proteins like other UBLs, the molecular mechanism of its attachment remains unknown. Here, we reconstitute the covalent attachment of thiocarboxylated Urm1 to various cellular target proteins in vitro, revealing that, unlike other known UBLs, this process is E2/E3-independent and requires oxidative stress. Furthermore, we present the crystal structures of the peroxiredoxin Ahp1 before and after the covalent attachment of Urm1. Surprisingly, we show that urmylation is accompanied by the transfer of sulfur to cysteine residues in the target proteins, also known as cysteine persulfidation. Our results illustrate the role of the Uba4-Urm1 system as a key evolutionary link between prokaryotic SCPs and the UBL modifications observed in modern eukaryotes. E2/E3-independent ubiquitin-like protein conjugation by Urm1 is directly coupled to cysteine persulfidation.,Ravichandran KE, Kaduhr L, Skupien-Rabian B, Shvetsova E, Sokolowski M, Krutyholowa R, Kwasna D, Brachmann C, Lin S, Guzman Perez S, Wilk P, Kosters M, Grudnik P, Jankowska U, Leidel SA, Schaffrath R, Glatt S EMBO J. 2022 Oct 17;41(20):e111318. doi: 10.15252/embj.2022111318. Epub 2022 Sep , 14. PMID:36102610 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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