7q82

Crystal structure of the methyltransferase-ribozyme 1, Thallium derivative (with 1-methyl-adenosine)

spinqualitylabelsall models 7q82, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7q82 is a 3 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.95Å Ligands: 1MA, GUN, MG, TL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed RNA-catalyzed RNA methylation was recently shown to be part of the catalytic repertoire of ribozymes. The methyltransferase ribozyme MTR1 catalyzes the site-specific synthesis of 1-methyladenosine (m(1)A) in RNA, using O(6)-methylguanine (m(6)G) as a methyl group donor. Here, we report the crystal structure of MTR1 at a resolution of 2.8 A, which reveals a guanine-binding site reminiscent of natural guanine riboswitches. The structure represents the postcatalytic state of a split ribozyme in complex with the m(1)A-containing RNA product and the demethylated cofactor guanine. The structural data suggest the mechanistic involvement of a protonated cytidine in the methyl transfer reaction. A synergistic effect of two 2'-O-methylated ribose residues in the active site results in accelerated methyl group transfer. Supported by these results, it seems plausible that modified nucleotides may have enhanced early RNA catalysis and that metabolite-binding riboswitches may resemble inactivated ribozymes that have lost their catalytic activity during evolution. Structure and mechanism of the methyltransferase ribozyme MTR1.,Scheitl CPM, Mieczkowski M, Schindelin H, Hobartner C Nat Chem Biol. 2022 May;18(5):547-555. doi: 10.1038/s41589-022-00976-x. Epub 2022, Mar 17. PMID:35301481 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribozyme 3D structures

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