7qi4

Human mitochondrial ribosome at 2.2 A resolution (bound to partly built tRNAs and mRNA)

spinqualitylabelsall models 7qi4, resolution 2.21Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qi4 is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.21Å Ligands: 1MA, 2MG, 5F0, 5MC, 5MU, ATP, AYA, B8T, FES, GDP, K, MA6, MG, NAD, OMG, OMU, PSU, PUT, SAC, SPD, SPM, THC, VAL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RT02_HUMAN Publication Abstract from PubMed The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 A resolution structure of human mitoribosome together with validated mitoribosomal RNA (rRNA) modifications, including aminoacylated CP-tRNA(Val). The structure shows how mitoribosomal proteins stabilise binding of mRNA and tRNA helping to align it in the decoding center, whereas the GDP-bound mS29 stabilizes intersubunit communication. Comparison between different states, with respect to tRNA position, allowed us to characterize a non-canonical L1 stalk, and molecular dynamics simulations revealed how it facilitates tRNA transitions in a way that does not require interactions with rRNA. We also report functionally important polyamines that are depleted when cells are subjected to an antibiotic treatment. The structural, biochemical, and computational data illuminate the principal functional components of the translation mechanism in mitochondria and provide a description of the structure and function of the human mitoribosome. Mitoribosome structure with cofactors and modifications reveals mechanism of ligand binding and interactions with L1 stalk.,Singh V, Itoh Y, Del'Olio S, Hassan A, Naschberger A, Flygaard RK, Nobe Y, Izumikawa K, Aibara S, Andrell J, Whitford PC, Barrientos A, Taoka M, Amunts A Nat Commun. 2024 May 20;15(1):4272. doi: 10.1038/s41467-024-48163-x. PMID:38769321 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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