7qim

In situ structure of nebulin bound to actin filament in skeletal sarcomere

spinqualitylabelsall models 7qim, resolution 4.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qim is a 7 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.5Å Ligands: ADP, HIC, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ACTS_MOUSE Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Publication Abstract from PubMed In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the underlying mechanism remains nebulous. In this work, we used cryo-electron tomography and subtomogram averaging to reveal structures of native nebulin bound to thin filaments within intact sarcomeres. This in situ reconstruction provided high-resolution details of the interaction between nebulin and actin, demonstrating the stabilizing role of nebulin. Myosin bound to the thin filaments exhibited different conformations of the neck domain, highlighting its inherent structural variability in muscle. Unexpectedly, nebulin did not interact with myosin or tropomyosin, but it did interact with a troponin T linker through two potential binding motifs on nebulin, explaining its regulatory role. Our structures support the role of nebulin as a thin filament "molecular ruler" and provide a molecular basis for studying nemaline myopathies. Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin.,Wang Z, Grange M, Pospich S, Wagner T, Kho AL, Gautel M, Raunser S Science. 2022 Feb 18;375(6582):eabn1934. doi: 10.1126/science.abn1934. Epub 2022 , Feb 18. PMID:35175800 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nebulin

Proteopedia Page Contributors and Editors (what is this?)

OCA