7qj6

Structure of recombinant human gamma-Tubulin Ring Complex 10-spoked assembly intermediate (spokes 3-12, substoichiometric spokes 13-14)

spinqualitylabelsall models 7qj6, resolution 7.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qj6 is a 31 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 7.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease ACTB_HUMAN Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [MIM:243310. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Function ACTB_HUMAN Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Publication Abstract from PubMed The gamma-tubulin ring complex (gamma-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the gamma-TuRC, containing more than thirty subunits, raising fundamental questions about gamma-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular gamma-TuRC assembly and identify a functional role of actin in microtubule nucleation. During gamma-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the gamma-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for gamma-TuRC assembly and structural integrity, but determines gamma-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo. Modular assembly of the principal microtubule nucleator gamma-TuRC.,Wurtz M, Zupa E, Atorino ES, Neuner A, Bohler A, Rahadian AS, Vermeulen BJA, Tonon G, Eustermann S, Schiebel E, Pfeffer S Nat Commun. 2022 Jan 25;13(1):473. doi: 10.1038/s41467-022-28079-0. PMID:35078983 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tubulin 3D Structures

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