7qla

Structure of the Rab GEF complex Mon1-Ccz1

spinqualitylabelsall models 7qla, resolution 3.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qla is a 2 chain structure with sequence from Chaetomium thermophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.85Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G0SGS3_CHATD Publication Abstract from PubMed Activation of the GTPase Rab7/Ypt7 by its cognate guanine nucleotide exchange factor (GEF) Mon1-Ccz1 marks organelles such as endosomes and autophagosomes for fusion with lysosomes/vacuoles and degradation of their content. Here, we present a high-resolution cryogenic electron microscopy structure of the Mon1-Ccz1 complex that reveals its architecture in atomic detail. Mon1 and Ccz1 are arranged side by side in a pseudo-twofold symmetrical heterodimer. The three Longin domains of each Mon1 and Ccz1 are triangularly arranged, providing a strong scaffold for the catalytic center of the GEF. At the opposite side of the Ypt7-binding site, a positively charged and relatively flat patch stretches the Longin domains 2/3 of Mon1 and functions as a phosphatidylinositol phosphate-binding site, explaining how the GEF is targeted to membranes. Our work provides molecular insight into the mechanisms of endosomal Rab activation and serves as a blueprint for understanding the function of members of the Tri Longin domain Rab-GEF family. Structure of the Mon1-Ccz1 complex reveals molecular basis of membrane binding for Rab7 activation.,Klink BU, Herrmann E, Antoni C, Langemeyer L, Kiontke S, Gatsogiannis C, Ungermann C, Raunser S, Kummel D Proc Natl Acad Sci U S A. 2022 Feb 8;119(6):e2121494119. doi: , 10.1073/pnas.2121494119. PMID:35105815 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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