7qvi

Fiber-forming RubisCO derived from ancestral sequence reconstruction and rational engineering

spinqualitylabelsall models 7qvi, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qvi is a 16 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CAP, KCX, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco. Evolution of increased complexity and specificity at the dawn of form I Rubiscos.,Schulz L, Guo Z, Zarzycki J, Steinchen W, Schuller JM, Heimerl T, Prinz S, Mueller-Cajar O, Erb TJ, Hochberg GKA Science. 2022 Oct 14;378(6616):155-160. doi: 10.1126/science.abq1416. Epub 2022, Oct 13. PMID:36227987 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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