7qx0

Transaminase Structure of Plurienzyme (Tr2E2) in complex with PLP

spinqualitylabelsall models 7qx0, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7qx0 is a 4 chain structure with sequence from Acidihalobacter. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Ligands: PLP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A3G5BC54_9GAMM Publication Abstract from PubMed Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area of enzyme engineering and catalysis. Herein we present the development of a PluriZyme, TR(2) E(2) , with efficient native transaminase (k(cat) : 69.49+/-1.77 min(-1) ) and artificial esterase (k(cat) : 3908-0.41 min(-1) ) activities integrated into a single scaffold, and evaluate its utility in a cascade reaction. TR(2) E(2) (pH(opt) : 8.0-9.5; T(opt) : 60-65 degrees C) efficiently converts methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate into 3-(R)-amino-4-(2,4,5-trifluorophenyl)butanoic acid, a crucial intermediate for the synthesis of antidiabetic drugs. The reaction proceeds through the conversion of the beta-keto ester into the beta-keto acid at the hydrolytic site and subsequently into the beta-amino acid (e.e. >99 %) at the transaminase site. The catalytic power of the TR(2) E(2) PluriZyme was proven with a set of beta-keto esters, demonstrating the potential of such designs to address bioinspired cascade reactions. A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.,Roda S, Fernandez-Lopez L, Benedens M, Bollinger A, Thies S, Schumacher J, Coscolin C, Kazemi M, Santiago G, Gertzen CGW, Gonzalez-Alfonso JL, Plou FJ, Jaeger KE, Smits SHJ, Ferrer M, Guallar V Angew Chem Int Ed Engl. 2022 Sep 12;61(37):e202207344. doi: , 10.1002/anie.202207344. Epub 2022 Aug 4. PMID:35734849 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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