7r0x

Structure of the branching thioesterase from oocydin biosynthesis

spinqualitylabelsall models 7r0x, resolution 2.83Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7r0x is a 1 chain structure with sequence from Serratia. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.83Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A318P693_SERPL Publication Abstract from PubMed Thioesterases (TEs) are fundamentally important enzymes present in all bacteria and eukaryotes, where they have conserved functions in fatty acid biosynthesis and secondary metabolism. This work provides the first structural insights into a functionally distinct group of TEs that perform diverse acylations in polyketide and peptide biosynthesis (TE(B) s). Structural analysis of the oocydin (OocS) TE(B) domain facilitated identification and engineering of the active site to modulate acyl-group acceptance. In this way, we achieved higher reactivity using a structure-based approach, building a foundation for biocatalytic development of TE(B) -mediated O-acylation, a modification known to improve the bioactivity of oocydin-type polyketides. Lastly, the promiscuity of the OocS TE(B) motivated us to investigate, and ultimately provide evidence for, the production of longer chain branched oocydins in the native host Serratia plymuthica 4Rx13. This work frames the OocS TE(B) and homologs as invaluable synthetic biology tools for polyketide drug development. Structure of a Promiscuous Thioesterase Domain Responsible for Branching Acylation in Polyketide Biosynthesis.,Fraley AE, Dieterich CL, Mabesoone MFJ, Minas HA, Meoded RA, Hemmerling F, Piel J Angew Chem Int Ed Engl. 2022 Sep 26;61(39):e202206385. doi: , 10.1002/anie.202206385. Epub 2022 Aug 19. PMID:35903999 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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