7r96

Self-Assembled 3D DNA Hexagonal Tensegrity Triangle

spinqualitylabelsall models 7r96, resolution 5.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7r96 is a 4 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 5.68Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The tensegrity triangle motif utilizes Watson-Crick sticky end cohesion to self-assemble into a rhombohedral crystal lattice using complementary 5'-GA and 5'-TC sticky ends. Here, we report that using noncanonical 5'-AG and 5'-TC sticky ends in otherwise isomorphic tensegrity triangles results in crystal self-assembly in the P6(3) hexagonal space group as revealed by X-ray crystallography. In this structure, the DNA double helices bend at the crossover positions, a feature that was not observed in the original design. Instead of propagating linearly, the tilt between base pairs of each right-handed helix results in a left-handed superstructure along the screw axis, forming a microtubule-like structure composed of three double helices with an unbroken channel at the center. This hexagonal lattice has a cavity diameter of 11 nm and a unit cell volume of 886 000 A(3)-far larger than the rhombohedral counterpart (5 nm, 330 000 A(3)). 3D Hexagonal Arrangement of DNA Tensegrity Triangles.,Lu B, Vecchioni S, Ohayon YP, Sha R, Woloszyn K, Yang B, Mao C, Seeman NC ACS Nano. 2021 Oct 26;15(10):16788-16793. doi: 10.1021/acsnano.1c06963. Epub 2021 , Oct 5. PMID:34609128 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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