7rbw

Structure of Biliverdin-binding Serpin of Boana punctata (polka-dot tree frog)

spinqualitylabelsall models 7rbw, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7rbw is a 2 chain structure with sequence from Boana punctata. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.05Å Ligands: BLA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A7D7FB99_BOAPU Publication Abstract from PubMed Biliverdin-binding serpins (BBSs) are proteins that are responsible for coloration in amphibians and fluoresce in the near-infrared (NIR) spectral region. Here we produced the first functional recombinant BBS of the polka-dot treefrog Boana punctata (BpBBS), assembled with its biliverdin (BV) chromophore, and report its biochemical and photochemical characterization. We determined the crystal structure of BpBBS at 2.05 A resolution, which demonstrated its structural homology to the mammalian protease inhibitor alpha-1-antitrypsin. BV interaction with BpBBS was studied and it was found that the N-terminal polypeptide (residues 19-50) plays a critical role in the BV binding. By comparing BpBBS with the available NIR fluorescent proteins and expressing it in mammalian cells, we demonstrated its potential as a NIR imaging probe. These results provide insight into the non-inhibitory function of serpins, provide a basis for improving their performance in mammalian cells, and suggest possible paths for the development of BBS-based fluorescent probes. Structural and Functional Characterization of a Biliverdin-Binding Near-Infrared Fluorescent Protein From the Serpin Superfamily.,Manoilov KY, Ghosh A, Almo SC, Verkhusha VV J Mol Biol. 2021 Nov 16;434(2):167359. doi: 10.1016/j.jmb.2021.167359. PMID:34798132 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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