7s0t
Structure of DNA polymerase zeta with mismatched DNA
Structural highlights
FunctionDPOZ_YEAST Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion.[1] [2] [3] [4] Publication Abstract from PubMedThe B-family multi-subunit DNA polymerase zeta (Polzeta) is important for translesion DNA synthesis (TLS) during replication, due to its ability to extend synthesis past nucleotides opposite DNA lesions and mismatched base pairs. We present a cryo-EM structure of Saccharomyces cerevisiae Polzeta with an A:C mismatch at the primer terminus. The structure shows how the Polzeta active site responds to the mismatched duplex DNA distortion, including the loosening of key protein-DNA interactions and a fingers domain in an "open" conformation, while the incoming dCTP is still able to bind for the extension reaction. The structure of the mismatched DNA-Polzeta ternary complex reveals insights into mechanisms that either stall or favor continued DNA synthesis in eukaryotes. Cryo-EM structure of translesion DNA synthesis polymerase zeta with a base pair mismatch.,Malik R, Johnson RE, Prakash L, Prakash S, Ubarretxena-Belandia I, Aggarwal AK Nat Commun. 2022 Feb 25;13(1):1050. doi: 10.1038/s41467-022-28644-7. PMID:35217661[5] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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