7sfn

Crystal structure of OlmO, a spirocyclase involved in the biosynthesis of oligomycin

spinqualitylabelsall models 7sfn, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7sfn is a 2 chain structure with sequence from Streptomyces avermitilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: MLT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q93HI7_STRAX Publication Abstract from PubMed A key step in the biosynthesis of numerous polyketides is the stereospecific formation of a spiroacetal (spiroketal). We report here that spiroacetal formation in the biosynthesis of the macrocyclic polyketides ossamycin and oligomycin involves catalysis by a novel spiroacetal cyclase. OssO from the ossamycin biosynthetic gene cluster (BGC) is homologous to OlmO, the product of an unannotated gene from the oligomycin BGC. The deletion of olmO abolished oligomycin production and led to the isolation of oligomycin-like metabolites lacking the spiroacetal structure. Purified OlmO catalyzed complete conversion of the major metabolite into oligomycin C. Crystal structures of OssO and OlmO reveal an unusual 10-strand beta-barrel. Three conserved polar residues are clustered together in the beta-barrel cavity, and site-specific mutation of any of these residues either abolished or substantially diminished OlmO activity, supporting a role for general acid/general base catalysis in spiroacetal formation. Enzyme-Catalyzed Spiroacetal Formation in Polyketide Antibiotic Biosynthesis.,Bilyk O, Oliveira GS, de Angelo RM, Almeida MO, Honorio KM, Leeper FJ, Dias MVB, Leadlay PF J Am Chem Soc. 2022 Aug 17;144(32):14555-14563. doi: 10.1021/jacs.2c03313. Epub , 2022 Aug 3. PMID:35921248 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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