7spu

In situ cryo-EM structure of bacteriophage Sf6 gp3:gp7:gp5 complex in conformation 1 at 3.73A resolution

spinqualitylabelsall models 7spu, resolution 3.73Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7spu is a 54 chain structure with sequence from Shigella virus Sf6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.73Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q716G8_BPSFV Publication Abstract from PubMed Sf6 is a bacterial virus that infects the human pathogen Shigella flexneri. Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6 degrees with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae. High-resolution cryo-EM structure of the Shigella virus Sf6 genome delivery tail machine.,Li F, Hou CD, Yang R, Whitehead R 3rd, Teschke CM, Cingolani G Sci Adv. 2022 Dec 9;8(49):eadc9641. doi: 10.1126/sciadv.adc9641. Epub 2022 Dec 7. PMID:36475795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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