7t1p

Solution structure of 7SK stem-loop 1 with HIV-1 Tat Finland Arginine Rich Motif

spinqualitylabelsall models 7t1p Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7t1p is a 2 chain structure with sequence from HIV-1 06TG.HT008 and Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Productive transcriptional elongation of many cellular and viral mRNAs requires transcriptional factors to extract pTEFb from the 7SK snRNP by modulating the association between HEXIM and 7SK snRNA. In HIV-1, Tat binds to 7SK by displacing HEXIM. However, without the structure of the 7SK-HEXIM complex, the constraints that must be overcome for displacement remain unknown. Furthermore, while structure details of the Tat(NL4-3)-7SK complex have been elucidated, it is unclear how subtypes with more HEXIM-like Tat sequences accomplish displacement. Here we report the structures of HEXIM, Tat(G), and Tat(Fin) arginine rich motifs in complex with the apical stemloop-1 of 7SK. While most interactions between 7SK with HEXIM and Tat are similar, critical differences exist that guide function. First, the conformational plasticity of 7SK enables the formation of three different base pair configurations at a critical remodeling site, which allows for the modulation required for HEXIM binding and its subsequent displacement by Tat. Furthermore, the specific sequence variations observed in various Tat subtypes all converge on remodeling 7SK at this region. Second, we show that HEXIM primes its own displacement by causing specific local destabilization upon binding - a feature that is then exploited by Tat to bind 7SK more efficiently. A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA.,Pham VV, Gao M, Meagher JL, Smith JL, D'Souza VM Commun Biol. 2022 Aug 15;5(1):819. doi: 10.1038/s42003-022-03734-w. PMID:35970937 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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