7twm | pdb_00007twm

Structure of a borosin methyltransferase from Mycena rosella with peptide CspL(MroMCspL) in complex with SAH

spinqualitylabelsall models 7twm, resolution 1.93Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7twm is a 4 chain structure with sequence from Mycena rosella. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.93Å Ligands: MLE, SAH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed N-methylation of peptide backbones has often been utilized as a strategy towards the development of peptidic drugs. However, difficulties in the chemical synthesis, high cost of enantiopure N-methyl building blocks, and subsequent coupling inefficiencies have hampered larger-scale medicinal chemical efforts. Here, we present a chemoenzymatic strategy for backbone N-methylation by bioconjugation of peptides of interest to the catalytic scaffold of a borosin-type methyltransferase. Crystal structures of a substrate tolerant enzyme from Mycena rosella guided the design of a decoupled catalytic scaffold that can be linked via a heterobifunctional crosslinker to any peptide substrate of choice. Peptides linked to the scaffold, including those with non-proteinogenic residues, show robust backbone N-methylation. Various crosslinking strategies were tested to facilitate substrate disassembly, which enabled a reversible bioconjugation approach that efficiently released modified peptide. Our results provide general framework for the backbone N-methylation on any peptide of interest and may facilitate the production of large libraries of N-methylated peptides. Bioconjugate Platform for Iterative Backbone N-Methylation of Peptides.,Zheng Y, Ongpipattanakul C, Nair SK ACS Catal. 2022 Nov 18;12(22):14006-14014. doi: 10.1021/acscatal.2c04681. Epub , 2022 Oct 31. PMID:36793448 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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