7uhl

Time-Resolved Structure of Metallo Beta-Lactamase L1 in a Complex with Non-Hydrolyzed Moxalactam (100 ms Snapshot)

spinqualitylabelsall models 7uhl, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7uhl is a 1 chain structure with sequence from Stenotrophomonas maltophilia K279a. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: MX0, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2FTM1_STRMK Publication Abstract from PubMed Serial x-ray crystallography can uncover binding events, and subsequent chemical conversions occurring during enzymatic reaction. Here, we reveal the structure, binding and cleavage of moxalactam antibiotic bound to L1 metallo-beta-lactamase (MBL) from Stenotrophomonas maltophilia. Using time-resolved serial synchrotron crystallography, we show the time course of beta-lactam hydrolysis and determine ten snapshots (20, 40, 60, 80, 100, 150, 300, 500, 2000 and 4000 ms) at 2.20 A resolution. The reaction is initiated by laser pulse releasing Zn(2+) ions from a UV-labile photocage. Two metal ions bind to the active site, followed by binding of moxalactam and the intact beta-lactam ring is observed for 100 ms after photolysis. Cleavage of beta-lactam is detected at 150 ms and the ligand is significantly displaced. The reaction product adjusts its conformation reaching steady state at 2000 ms corresponding to the relaxed state of the enzyme. Only small changes are observed in the positions of Zn(2+) ions and the active site residues. Mechanistic details captured here can be generalized to other MBLs. Time-resolved beta-lactam cleavage by L1 metallo-beta-lactamase.,Wilamowski M, Sherrell DA, Kim Y, Lavens A, Henning RW, Lazarski K, Shigemoto A, Endres M, Maltseva N, Babnigg G, Burdette SC, Srajer V, Joachimiak A Nat Commun. 2022 Nov 30;13(1):7379. doi: 10.1038/s41467-022-35029-3. PMID:36450742 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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