7uoi

Crystallographic structure of DapE from Enterococcus faecium

spinqualitylabelsall models 7uoi, resolution 1.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7uoi is a 1 chain structure with sequence from Enterococcus faecium 1,231,410. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.6Å Ligands: GOL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1S8KJG1_ENTFC Publication Abstract from PubMed DapE is a Zn(2+)-metallohydrolase recognized as a drug target for bacterial control. It is a homodimer that requires the exchange of interface strands by an induced fit essential for catalysis. Identifying novel anti-DapE agents requires greater structural details. Most of the characterized DapEs are from the Gram-negative group. Here, two high-resolution DapE crystal structures from Enterococcus faecium are presented for the first time with novel aspects. A loosened enzyme intermediate between the open and closed conformations is observed. Substrates may bind to loose state, subsequently it closes, where hydrolysis occurs, and finally, the change to the open state leads to the release of the products. Mutation of His352 suggests a role, along with His194, in the oxyanion stabilization in the mono-metalated Zn(2+) isoform, while in the di-metalated isoform, the metal center 2 complements it function. An aromatic-pi box potentially involved in the interaction of DapE with other proteins, and a peptide flip could determine the specificity in the Gram-positive ArgE/DapE group. Finally, details of two extra-catalytic cavities whose geometry changes depending on the conformational state of the enzyme are presented. These cavities could be a target for developing non-competitive agents that trap the enzyme in an inactive state. The three-dimensional structure of DapE from Enterococcus faecium reveals new insights into DapE/ArgE subfamily ligand specificity.,Terrazas-Lopez M, Gonzalez-Segura L, Diaz-Vilchis A, Aguirre-Mendez KA, Lobo-Galo N, Martinez-Martinez A, Diaz-Sanchez AG Int J Biol Macromol. 2024 May 11;270(Pt 2):132281. doi: , 10.1016/j.ijbiomac.2024.132281. PMID:38740150 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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