7upv

Structure of maize BZR1-type beta-amylase provides new insights into its noncatalytic adaptation

spinqualitylabelsall models 7upv, resolution 1.84Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7upv is a 1 chain structure with sequence from Zea mays. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.84Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1D6QTZ9_MAIZE Publication Abstract from PubMed Plant beta-amylase (BAM) proteins play an essential role in growth, development, stress response, and hormone regulation. Despite their typical (beta/alpha)(8) barrel structure as active catalysts in starch breakdown, catalytically inactive BAMs are implicated in diverse yet elusive functions in plants. The noncatalytic BAM7/8 contain N-terminal BZR1 domains and were shown to be involved in the regulation of brassinosteroid signaling and possibly serve as sensors of yet an uncharacterized metabolic signal. While the structures of several catalytically active BAMs have been reported, structural characterization of the catalytically inactive BZR1-type BAMs remain unknown. Here, we determine the crystal structure of beta-amylase domain of Zea mays BAM8/BES1/BZR1-5 and provide comprehensive insights into its noncatalytic adaptation. Using structural-guided comparison combined with biochemical analysis and molecular dynamics simulations, we revealed conformational changes in multiple distinct highly conserved regions resulting in rearrangement of the binding pocket. Altogether, this study adds a new layer of understanding to starch breakdown mechanism and elucidates the acquired adjustments of noncatalytic BZR1-type BAMs as putative regulatory domains and/or metabolic sensors in plants. Structure of maize BZR1-type beta-amylase BAM8 provides new insights into its noncatalytic adaptation.,Sun F, Palayam M, Shabek N J Struct Biol. 2022 Sep;214(3):107885. doi: 10.1016/j.jsb.2022.107885. Epub 2022 , Aug 10. PMID:35961473 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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