7v6m

Crystal structure of lacto-N-biosidase TnX from Tynzenella nexilis, lacto-N-biose complex

spinqualitylabelsall models 7v6m, resolution 1.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7v6m is a 1 chain structure with sequence from Tyzzerella nexilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.92Å Ligands: GAL, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Glycoside hydrolase family 136 (GH136) was established after the discovery and structural analysis of lacto-N-biosidase (LNBase) from the infant gut bacterium Bifidobacterium longum subsp. longum JCM1217 (BlLnbX). Homologous genes of BlLnbX are widely distributed in the genomes of human gut bacteria and monkey Bifidobacterium spp., although only two crystal structures were reported in the GH136 family. Cell suspensions of Bifidobacterium saguini, Tyzzerella nexilis, and Ruminococcus lactaris exhibited the LNBase activity. Recombinant LNBases of these three species were functionally expressed with their specific chaperones in Escherichia coli, and their kinetic parameters against p-nitrophenol substrates were determined. The crystal structures of the LNBases from B. saguini and T. nexilis in complex with lacto-N-biose I were determined at 2.5 A and 1.9 A resolutions, respectively. These structures conserve a beta-helix fold characteristic of GH136 and the catalytic residues, but they lack the metal ions that were present in BlLnbX. Crystal structures of glycoside hydrolase family 136 lacto-N-biosidases from monkey gut- and human adult gut bacteria.,Yamada C, Katayama T, Fushinobu S Biosci Biotechnol Biochem. 2022 Jan 29. pii: 6517306. doi: 10.1093/bbb/zbac015. PMID:35092420 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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