7v9x

Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.8 angstrom

spinqualitylabelsall models 7v9x, resolution 2.82Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7v9x is a 9 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.82Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RT86_ECOLX Reverse transcriptase (RT) component of antiviral defense system retron Ec86, composed of a non-coding RNA (ncRNA), a ribosyltransferase/DNA-binding protein and this RT. Expression of the 3-gene retron confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:10531319, PubMed:2466573). Recognizes only its cognate RNA as a primer template (PubMed:10531319). Overexpression of the ncRNA and RT (without the ribosyltransferase), which leads to increased levels of msDNA, is mutagenic in vivo (PubMed:7885227). This may be due to a mismatch in the msDNA stem which binds and sequesters MutS and/or MutL (Probable).

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