7vs9

Crystal structure of P domain from norovirus GI.9 capsid protein in complex with Lewis x antigen.

spinqualitylabelsall models 7vs9, resolution 2.26Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7vs9 is a 2 chain structure with sequence from Norovirus Hu/GI/Vancouver730/2004/CAN. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.26Å Ligands: CL, FUC, GAL, MG, NDG, PRD_900120 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F2XMU3_9CALI Publication Abstract from PubMed Noroviruses have been identified as major causative agents of acute nonbacterial gastroenteritis in humans. Histo-blood group antigens (HBGAs) are thought to play a major role among the host cellular factors influencing norovirus infection. Genogroup I, genotype 9 (GI.9) is the most recently identified genotype within genogroup I, whose representative strain is the Vancouver 730 norovirus. However, the molecular interactions between host antigens and the GI.9 capsid protein have not been investigated in detail. In this study, we demonstrate that the GI.9 norovirus preferentially binds Lewis antigens over blood group A, B, and H antigens, as revealed by an HBGA binding assay using virus-like particles. We determined the crystal structures of the protruding domain of the GI.9 capsid protein in the presence or absence of Lewis antigens. Our analysis demonstrated that Lewis fucose (alpha1-3/4 fucose) represents a key moiety for the GI.9 protein-HBGA interaction, thus suggesting that Lewis antigens might play a critical role during norovirus infection. In addition to previously reported findings, our observations may support the future design of antiviral agents and vaccines against noroviruses. Lewis fucose is a key moiety for the recognition of histo-blood group antigens by GI.9 norovirus, as revealed by structural analysis.,Kimura-Someya T, Kato-Murayama M, Katsura K, Sakai N, Murayama K, Hanada K, Shirouzu M, Someya Y FEBS Open Bio. 2022 Mar;12(3):560-570. doi: 10.1002/2211-5463.13370. Epub 2022, Jan 30. PMID:35038379 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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