7vwv

The crystal structure of African swine fever virus I73R

spinqualitylabelsall models 7vwv, resolution 2.59Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7vwv is a 2 chain structure with sequence from African swine fever virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.59Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2X0RU36_ASF Publication Abstract from PubMed African swine fever virus (ASFV) is a large, double-stranded DNA virus that causes a fatal disease in pigs, posing a threat to the global pig industry. Whereas some ASFV proteins have been found to play important roles in ASFV-host interaction, the functional roles of many proteins are still largely unknown. In this study, we identified I73R, an early viral gene in the replication cycle of ASFV, as a key virulence factor. Our findings demonstrate that pI73R suppresses the host innate immune response by broadly inhibiting the synthesis of host proteins, including antiviral proteins. Crystallization and structural characterization results suggest that pI73R is a nucleic-acid-binding protein containing a Zalpha domain. It localizes in the nucleus and inhibits host protein synthesis by suppressing the nuclear export of cellular messenger RNA (mRNAs). While pI73R promotes viral replication, the deletion of the gene showed that it is a nonessential gene for virus replication. In vivo safety and immunogenicity evaluation results demonstrate that the deletion mutant ASFV-GZDeltaI73R is completely nonpathogenic and provides effective protection to pigs against wild-type ASFV. These results reveal I73R as a virulence-related gene critical for ASFV pathogenesis and suggest that it is a potential target for virus attenuation. Accordingly, the deletion mutant ASFV-GZDeltaI73R can be a potent live-attenuated vaccine candidate. African swine fever virus I73R is a critical virulence-related gene: A potential target for attenuation.,Liu Y, Shen Z, Xie Z, Song Y, Li Y, Liang R, Gong L, Di D, Liu J, Liu J, Chen Z, Yu W, Lv L, Zhong Q, Liao X, Tian C, Wang R, Song Q, Wang H, Peng G, Chen H Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2210808120. doi: , 10.1073/pnas.2210808120. Epub 2023 Apr 6. PMID:37023125 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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