7w0v

C4'-SCF3-DT modifeid DNA-DNA duplex

spinqualitylabelsall models 7w0v Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7w0v is a 2 chain structure with sequence from Octodon degus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 84E Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Fluorinated nucleotides are invaluable for (19) F NMR studies of nucleic acid structure and function. Here, we synthesized 4'-SCF3 -thymidine (T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ ) and incorporated it into DNA by means of solid-phase DNA synthesis. NMR studies showed that the 4'-SCF3 group exhibited a flexible orientation in the minor groove of DNA duplexes and was well accommodated by various higher order DNA structures. The three magnetically equivalent fluorine atoms in 4'-SCF3 -DNA constitute an isolated spin system, offering high (19) F NMR sensitivity and excellent resolution of the positioning of T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ within various secondary and tertiary DNA structures. The high structural adaptability and high sensitivity of T 4 ' - SCF 3 ${{^{4{^\prime}\hbox{-}{\rm SCF}{_{3}}}}}$ make it a valuable (19) F NMR probe for quantitatively distinguishing diverse DNA structures with single-nucleotide resolution and for monitoring the dynamics of interactions in the minor groove of double-stranded DNA. 4'-SCF3 -Labeling Constitutes a Sensitive (19) F NMR Probe for Characterization of Interactions in the Minor Groove of DNA.,Li Q, Trajkovski M, Fan C, Chen J, Zhou Y, Lu K, Li H, Su X, Xi Z, Plavec J, Zhou C Angew Chem Int Ed Engl. 2022 Nov 21;61(47):e202201848. doi:, 10.1002/anie.202201848. Epub 2022 Oct 19. PMID:36163470 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA