7w12

Complex structure of alginate lyase AlyB-OU02 with G9

spinqualitylabelsall models 7w12, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7w12 is a 1 chain structure with sequence from Vibrio splendidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Ligands: LGU, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Alginate lyases can be used to produce well-defined alginate oligosaccharides (AOSs) because of their specificities for AOS products. A large number of alginate lyases have been recorded in the CAZy database; however, the majority are annotated-only alginate lyases that include little information on their products, thus limiting their applications. Here, we establish a simple and experiment-saving approach to predict product distributions for PL7 alginate lyases through extensive structural biology, bioinformatics and biochemical studies. Structural study on several PL7 alginate lyases reveals that two loops around the substrate binding cleft determine product distribution. Furthermore, a database containing the loop information of all annotated-only single-domain PL7 alginate lyases is constructed, enabling systematic exploration of the association between loop and product distribution. Based on these results, a simplified loop/product distribution relationship is proposed, giving us information on product distribution directly from the amino acid sequence. Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.,Zhang K, Li Z, Zhu Q, Cao H, He X, Zhang XH, Liu W, Lyu Q Commun Biol. 2022 Aug 2;5(1):782. doi: 10.1038/s42003-022-03721-1. PMID:35918517 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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