7wm5

Crystal structure of apo TrmM from Mycoplasma capricolum

spinqualitylabelsall models 7wm5, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7wm5 is a 1 chain structure with sequence from Mycoplasma capricolum subsp. capricolum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.15Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0C2W699_MYCCA Publication Abstract from PubMed N(6) -methyladenosine (m(6) A), widely distributed in both coding and noncoding RNAs, regulates the epigenetic signals and RNA metabolism in eukaryotes. Although this posttranscriptional modification is frequently observed in messenger and ribosomal RNA, it is relatively rare in transfer RNA. In Escherichia coli, TrmM encoded by yfiC is the tRNA-specific N(6) methyltransferase, which modifies the A37 residue of tRNA(Val) (cmo(5) UAC) using S-adenosyl-l-methionine as a methyl donor. However, the structure-function relationship of this enzyme is not completely understood. In this report, we determined two x-ray crystal structures of Mycoplasma capricolum TrmM with and without S-adenosyl-l-homocysteine, which is a reaction product. We also demonstrated the cellular and in vitro activities of this enzyme in the m(6) A modification of tRNA and the requirement of a divalent metal ion for its function, which is unprecedented in other RNA N(6) methyltransferases, including the E. coli TrmM. Our results reveal that the dimeric form of M. capricolum TrmM is important for efficient tRNA binding and catalysis, thereby offering insights into the distinct substrate specificity of the monomeric E. coli homolog. Structural and functional characterization of TrmM in m(6) A modification of bacterial tRNA.,Jeong H, Lee Y, Kim J Protein Sci. 2022 May;31(5):e4319. doi: 10.1002/pro.4319. PMID:35481631 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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