7wyl

Structure of the EV71 3Cpro with 337 inhibitor

spinqualitylabelsall models 7wyl, resolution 1.31Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7wyl is a 2 chain structure with sequence from Enterovirus A71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.31Å Ligands: G7L Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E7E815_HE71 Publication Abstract from PubMed RNA viruses are critically dependent upon virally encoded proteases to cleave the viral polyproteins into functional proteins. Many of these proteases exhibit a similar fold and contain an essential catalytic cysteine, offering the opportunity to inhibit these enzymes with electrophilic small molecules. Here we describe the successful application of quantitative irreversible tethering (qIT) to identify acrylamide fragments that target the active site cysteine of the 3C protease (3C(pro)) of Enterovirus 71, the causative agent of hand, foot and mouth disease in humans, altering the substrate binding region. Further, we re-purpose these hits towards the main protease (M(pro)) of SARS-CoV-2 which shares the 3C-like fold and a similar active site. The hit fragments covalently link to the catalytic cysteine of M(pro) to inhibit its activity. We demonstrate that targeting the active site cysteine of M(pro) can have profound allosteric effects, distorting secondary structures to disrupt the active dimeric unit. Acrylamide fragment inhibitors that induce unprecedented conformational distortions in enterovirus 71 3C and SARS-CoV-2 main protease.,Qin B, Craven GB, Hou P, Chesti J, Lu X, Child ES, Morgan RML, Niu W, Zhao L, Armstrong A, Mann DJ, Cui S Acta Pharm Sin B. 2022 Oct;12(10):3924-3933. doi: 10.1016/j.apsb.2022.06.002. , Epub 2022 Jun 9. PMID:35702321 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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