7x9s

Crystal structure of a complex between the antirepressor GmaR and the transcriptional repressor MogR

spinqualitylabelsall models 7x9s, resolution 3.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7x9s is a 6 chain structure with sequence from Listeria monocytogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.11Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A3A6YDN3_LISMN Publication Abstract from PubMed The pathogenic Listeria monocytogenes bacterium produces the flagellum as a locomotive organelle at or below 30 degrees C outside the host, but it halts flagellar expression at 37 degrees C inside the human host to evade the flagellum-induced immune response. Listeria monocytogenes GmaR is a thermosensor protein that coordinates flagellar expression by binding the master transcriptional repressor of flagellar genes (MogR) in a temperature-responsive manner. To understand the regulatory mechanism whereby GmaR exerts the antirepression activity on flagellar expression, we performed structural and mutational analyses of the GmaR-MogR system. At or below 30 degrees C, GmaR exists as a functional monomer and forms a circularly enclosed multidomain structure via an interdomain interaction. GmaR in this conformation recognizes MogR using the C-terminal antirepressor domain in a unique dual binding mode and mediates the antirepressor function through direct competition and spatial restraint mechanisms. Surprisingly, at 37 degrees C, GmaR rapidly forms autologous aggregates that are deficient in MogR neutralization capabilities. Structural basis of flagellar motility regulation by the MogR repressor and the GmaR antirepressor in Listeria monocytogenes.,Cho SY, Na HW, Oh HB, Kwak YM, Song WS, Park SC, Jeon WJ, Cho H, Oh BC, Park J, Kang SG, Lee GS, Yoon SI Nucleic Acids Res. 2022 Oct 28;50(19):11315-11330. doi: 10.1093/nar/gkac815. PMID:36283692 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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