7xkz

Solution structure of subunit epsilon of the Mycobacterium abscessus F-ATP synthase

spinqualitylabelsall models 7xkz Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7xkz is a 1 chain structure with sequence from Mycobacteroides abscessus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0U0YP27_9MYCO Produces ATP from ADP in the presence of a proton gradient across the membrane.[HAMAP-Rule:MF_00530] Publication Abstract from PubMed Mycobacterium abscessus (Mab) is a nontuberculous mycobacterium of increasing clinical relevance. The rapidly growing opportunistic pathogen is intrinsically multi-drug-resistant and causes difficult-to-cure lung disease. Adenosine triphosphate, generated by the essential F(1) F(O) ATP synthase, is the major energy currency of the pathogen, bringing this enzyme complex into focus for the discovery of novel antimycobacterial compounds. Coupling of proton translocation through the membrane-embedded F(O) sector and ATP formation in the F(1) headpiece of the bipartite F(1) F(O) ATP synthase occurs via the central stalk subunits gamma and epsilon. Here, we used solution NMR spectroscopy to resolve the first atomic structure of the Mab subunit epsilon (Mabepsilon), showing that it consists of an N-terminal beta-barrel domain (NTD) and a helix-loop-helix motif in its C-terminal domain (CTD). NMR relaxation measurements of Mabepsilon shed light on dynamic epitopes and amino acids relevant for coupling processes within the protein. We describe structural differences between other mycobacterial epsilon subunits and Mabepsilon's lack of ATP binding. Based on the structural insights, we conducted an in silico inhibitor screen. One hit, Ep1MabF1, was shown to inhibit the growth of Mab and bacterial ATP synthesis. NMR titration experiments and docking studies described the binding epitopes of Ep1MabF1 on Mabepsilon. Together, our data demonstrate the potential to develop inhibitors targeting the epsilon subunit of Mab F(1) F(O) ATP synthase to interrupt the coupling process. Atomic solution structure of Mycobacterium abscessus F-ATP synthase subunit epsilon and identification of Ep1MabF1 as a targeted inhibitor.,Shin J, Harikishore A, Wong CF, Ragunathan P, Dick T, Gruber G FEBS J. 2022 Oct;289(20):6308-6323. doi: 10.1111/febs.16536. Epub 2022 Jun 5. PMID:35612822 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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