7xrt

Bacteroides thetaiotaomicron ferulic acid esterase (BT_4077)

spinqualitylabelsall models 7xrt, resolution 2.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7xrt is a 10 chain structure with sequence from Bacteroides thetaiotaomicron VPI-5482. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.007Å Ligands: CA, MPD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8A0E4_BACTN Publication Abstract from PubMed Oligomeric feruloyl esterase (FAE) has great application prospect in industry due to its potentially high stability and fine-tuned activity. However, the relationship between catalytic capability and oligomeric structure remains undetermined. Here we identified and characterized a novel, cold-adapted FAE (BtFae) derived from Bacteroides thetaiotaomicron. Structural studies unraveled that BtFae adopts a barrel-like decameric architecture unique in esterase families. By disrupting the interface, the monomeric variant exhibited significantly reduced catalytic activity and stability toward methyl ferulate, potentially due to its impact on the flexibility of the catalytic triad. Additionally, our results also showed that the monomerization of BtFae severely decreased the ferulic acid release from de-starched wheat bran and insoluble wheat arabinoxylan by 75 % and 80 %, respectively. Collectively, this study revealed novel connections between oligomerization and FAE catalytic function, which will benefit for further protein engineering of FAEs at the quaternary structure level for improved industrial applications. Structural insights into the oligomeric effects on catalytic activity of a decameric feruloyl esterase and its application in ferulic acid production.,Du G, Wang Y, Zhang Y, Yu H, Liu S, Ma X, Cao H, Wei X, Wen B, Li Z, Fan S, Zhou H, Xin F Int J Biol Macromol. 2023 Dec 31;253(Pt 1):126540. doi: , 10.1016/j.ijbiomac.2023.126540. Epub 2023 Aug 25. PMID:37634773 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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