Structural highlights
FunctionYBEY_STAAM Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Publication Abstract from PubMedEndoribonuclease YbeY is specific to the single-stranded RNA of ribosomal RNAs and small RNAs. This enzyme is essential for the maturation and quality control of ribosomal RNA in a wide range of bacteria and for virulence in some pathogenic bacteria. In this study, we determined the crystal structure of YbeY from Staphylococcus aureus at a resolution of 1.9 A in the presence of zinc chloride. The structure showed a zinc ion at the active site and two molecules of tricarboxylic acid citrate, which were also derived from the crystallization conditions. Our structure showed the zinc ion-bound local environment at the molecular level for the first time. Molecular comparisons were performed between the carboxylic moieties of citrate and the phosphate moiety of the RNA backbone, and a model of YbeY in complex with a single strand of RNA was subsequently constructed. Our findings provide molecular insights into how the YbeY enzyme recognizes single-stranded RNA in bacteria. Crystal Structure of the Metallo-Endoribonuclease YbeY from Staphylococcus aureus.,Lee J, Jo I, Kwon AR, Ha NC J Microbiol Biotechnol. 2023 Jan 28;33(1):28-34. doi: 10.4014/jmb.2209.09019. , Epub 2022 Nov 23. PMID:36457189[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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