7y87

class I diterpene synthase mutant (CyS-C59A) from Streptomyces cattleya

spinqualitylabelsall models 7y87, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7y87 is a 1 chain structure with sequence from Streptomyces cattleya. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F8JK18_STREN Publication Abstract from PubMed The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS(C59A) variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes. Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.,Xing B, Xu H, Li A, Lou T, Xu M, Wang K, Xu Z, Dickschat JS, Yang D, Ma M Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202209785. doi: , 10.1002/anie.202209785. Epub 2022 Aug 1. PMID:35819825 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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