7yoj

Structure of CasPi with guide RNA and target DNA

spinqualitylabelsall models 7yoj, resolution 3.36Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7yoj is a 4 chain structure with sequence from Armatimonadetes bacterium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.36Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A399WQY8_UNCAM Publication Abstract from PubMed CRISPR-Cas modules serve as the adaptive nucleic acid immune systems for prokaryotes, and provide versatile tools for nucleic acid manipulation in various organisms. Here, we discovered a new miniature type V system, CRISPR-Caspi (Cas12l) (~860 aa), from the environmental metagenome. Complexed with a large guide RNA (~170 nt) comprising the tracrRNA and crRNA, Caspi (Cas12l) recognizes a unique 5' C-rich PAM for DNA cleavage under a broad range of biochemical conditions, and generates gene editing in mammalian cells. Cryo-EM study reveals a 'bracelet' architecture of Caspi effector encircling the DNA target at 3.4 A resolution, substantially different from the canonical 'two-lobe' architectures of Cas12 and Cas9 nucleases. The large guide RNA serves as a 'two-arm' scaffold for effector assembly. Our study expands the knowledge of DNA targeting mechanisms by CRISPR effectors, and offers an efficient but compact platform for DNA manipulation. The compact Caspi (Cas12l) 'bracelet' provides a unique structural platform for DNA manipulation.,Sun A, Li CP, Chen Z, Zhang S, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J, Liu JG Cell Res. 2023 Mar;33(3):229-244. doi: 10.1038/s41422-022-00771-2. Epub 2023 Jan , 17. PMID:36650285 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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