7ywf

Monocot chimeric jacalin JAC1 from Oryza sativa: dirigent domain with bound galactobiose

spinqualitylabelsall models 7ywf, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7ywf is a 3 chain structure with sequence from Oryza sativa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: DTU, GAL, PO4, PRD_900113, TRS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q306J3_ORYSJ Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism.[RuleBase:RU363099] Publication Abstract from PubMed Pesticides are routinely used to prevent severe losses in agriculture. This practice is under debate because of its potential negative environmental impact and selection of resistances in pathogens. Therefore, the development of disease resistant plants is mandatory. It was shown that the rice (Oryza sativa) protein OsJAC1 enhances resistance against different bacterial and fungal plant pathogens in rice, barley, and wheat. Recently we reported possible carbohydrate interaction partners for both domains of OsJAC1 (a jacalin-related lectin (JRL) and a dirigent (DIR) domain), however, a mechanistic understanding of its function is still lacking. Here, we report crystal structures for both individual domains and the complex of galactobiose with the DIR domain, which revealed a new carbohydrate binding motif for DIR proteins. Docking studies of the two domains led to a model of the full-length protein. Our findings offer insights into structure and binding properties of OsJAC1 and its possible function in pathogen resistance. The Crystal Structure of the Defense Conferring Rice Protein OsJAC1 Reveals a Carbohydrate Binding Site on the Dirigent-like Domain.,Huwa N, Weiergraber OH, Fejzagic AV, Kirsch C, Schaffrath U, Classen T Biomolecules. 2022 Aug 17;12(8). pii: biom12081126. doi: 10.3390/biom12081126. PMID:36009020 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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