7zg5

The crystal structure of Salmonella TacAT3-DNA complex

spinqualitylabelsall models 7zg5, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7zg5 is a 6 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: BA, COA, GOL, MPD Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TACT3_SALT1 Toxic component of a type II toxin-antitoxin (TA) system (PubMed:29777131, PubMed:34556858). Acetylates tRNA and inhibits translation (PubMed:29777131). Acetylates only Gly-tRNA on all 3 Gly-tRNA(Gly) isoacceptors in situ (PubMed:35609997). In vitro acetylates mainly Ile/Leu and Gly (PubMed:29777131). Overexpression during the lag phase of a tacA3-tacT3 deletion strain leads to a 150-fold increase in persister cells in the presence of cefotaxime and a non-growth state in the absence of antibiotic (PubMed:29777131). Persister cell formation and the growth defect are neutralized by cognate antitoxin TacA3, but not by TacA1 or TacA2 (PubMed:29777131, PubMed:34556858). Plays a role in persister cell formation (PubMed:24408438). The TacA3-TacT3 complex both represses and derepresses expression of its own operon (PubMed:38538913). The hexameric 4:2 TacA3-TacT3 complex binds promoter DNA and represses its transcription; both subunits are required (PubMed:38538913). The octomeric 4:4 TacA3-TacT3 complex derepresses the operon (PubMed:38538913). The shift from hexameric to octomeric complex probably alters DNA-binding, leading to dissociation from the operator DNA and derepression (Probable) (PubMed:38538913).

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