8acc

CryoEM structure of sweet potato mild mottle virus VLP

spinqualitylabelsall models 8acc, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8acc is a 2 chain structure with sequence from Ipomoea batatas and Nicotiana tabacum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q599X9_9POTY Publication Abstract from PubMed Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus, family Potyviridae, sharing Ipomoea batatas as common host, but transmitted, respectively, by aphids and whiteflies. Virions of family members consist of flexuous rods with multiple copies of a single coat protein (CP) surrounding the RNA genome. Here we report the generation of virus-like particles (VLPs) by transient expression of the CPs of SPFMV and SPMMV in the presence of a replicating RNA in Nicotiana benthamiana. Analysis of the purified VLPs by cryo-electron microscopy, gave structures with resolutions of 2.6 and 3.0 A, respectively, showing a similar left-handed helical arrangement of 8.8 CP subunits per turn with the C-terminus at the inner surface and a binding pocket for the encapsidated ssRNA. Despite their similar architecture, thermal stability studies reveal that SPMMV VLPs are more stable than those of SPFMV. CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.,Chase O, Javed A, Byrne MJ, Thuenemann EC, Lomonossoff GP, Ranson NA, Lopez-Moya JJ Commun Biol. 2023 Apr 19;6(1):433. doi: 10.1038/s42003-023-04799-x. PMID:37076658 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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