8axa

Cryo-EM structure of shCas12k-sgRNA-dsDNA ternary complex (type V-K CRISPR-associated transposon)

spinqualitylabelsall models 8axa, resolution 2.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8axa is a 4 chain structure with sequence from Scytonema hofmannii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.96Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A8X6EH11_9CYAN Publication Abstract from PubMed CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opt CRISPR-Cas systems for RNA-guided DNA transposition. CASTs integrate large DNA cargos into the attachment (att) site independently of homology-directed repair and thus hold promise for eukaryotic genome engineering. However, the functional diversity and complexity of CASTs hinder an understanding of their mechanisms. Here, we present the high-resolution cryoelectron microscopy (cryo-EM) structure of the reconstituted approximately 1 MDa post-transposition complex of the type V-K CAST, together with different assembly intermediates and diverse TnsC filament lengths, thus enabling the recapitulation of the integration complex formation. The results of mutagenesis experiments probing the roles of specific residues and TnsB-binding sites show that transposition activity can be enhanced and suggest that the distance between the PAM and att sites is determined by the lengths of the TnsB C terminus and the TnsC filament. This singular model of RNA-guided transposition provides a foundation for repurposing the system for genome-editing applications. Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.,Tenjo-Castano F, Sofos N, Stutzke LS, Temperini P, Fuglsang A, Pape T, Mesa P, Montoya G Mol Cell. 2024 Jun 20;84(12):2353-2367.e5. doi: 10.1016/j.molcel.2024.05.005. , Epub 2024 Jun 3. PMID:38834066 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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