8b5r

p97-p37-SPI substrate complex

spinqualitylabelsall models 8b5r, resolution 6.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8b5r is a 11 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PP1R7_HUMAN Regulatory subunit of protein phosphatase 1. Publication Abstract from PubMed The AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is unclear. Here, we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by extracting an inhibitory subunit from PP1. We show that PP1 and its partners SDS22 and inhibitor-3 (I3) are loaded tightly onto p97, surprisingly via a direct contact of SDS22 with the p97 N-domain. Loading is assisted by the p37 adapter that bridges two adjacent p97 N-domains underneath the substrate complex. A stretch of I3 is threaded into the central channel of the spiral-shaped p97 hexamer, while other elements of I3 are still attached to PP1. Thus, our data show how p97 arranges a protein complex between the p97 N-domain and central channel, suggesting a hold-and-extract mechanism for p97-mediated disassembly. Structural basis of ubiquitin-independent PP1 complex disassembly by p97.,van den Boom J, Marini G, Meyer H, Saibil HR EMBO J. 2023 Jul 17;42(14):e113110. doi: 10.15252/embj.2022113110. Epub 2023 Jun , 2. PMID:37264685 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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