8b6f

Cryo-EM structure of NADH:ubiquinone oxidoreductase (complex-I) from respiratory supercomplex of Tetrahymena thermophila

spinqualitylabelsall models 8b6f, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8b6f is a 10 chain structure with sequence from Tetrahymena thermophila SB210. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.8Å Ligands: 3PE, 8Q1, ADP, CDL, FES, FMN, LPP, MG, NDP, PC1, UDP, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q22E24_TETTS Publication Abstract from PubMed Mitochondrial energy conversion requires an intricate architecture of the inner mitochondrial membrane(1). Here we show that a supercomplex containing all four respiratory chain components contributes to membrane curvature induction in ciliates. We report cryo-electron microscopy and cryo-tomography structures of the supercomplex that comprises 150 different proteins and 311 bound lipids, forming a stable 5.8-MDa assembly. Owing to subunit acquisition and extension, complex I associates with a complex IV dimer, generating a wedge-shaped gap that serves as a binding site for complex II. Together with a tilted complex III dimer association, it results in a curved membrane region. Using molecular dynamics simulations, we demonstrate that the divergent supercomplex actively contributes to the membrane curvature induction and tubulation of cristae. Our findings highlight how the evolution of protein subunits of respiratory complexes has led to the I-II-III(2)-IV(2) supercomplex that contributes to the shaping of the bioenergetic membrane, thereby enabling its functional specialization. Structural basis of mitochondrial membrane bending by the I-II-III(2)-IV(2) supercomplex.,Muhleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Marechal A, Sharma V, Amunts A Nature. 2023 Mar;615(7954):934-938. doi: 10.1038/s41586-023-05817-y. Epub 2023 , Mar 22. PMID:36949187 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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