8b7o

X-ray structure of Auranofin-human H-chain ferritin

spinqualitylabelsall models 8b7o, resolution 1.17Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8b7o is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.17Å Ligands: AU, CL, FE, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FRIH_HUMAN Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Publication Abstract from PubMed Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain. Evaluation of Auranofin Loading within Ferritin Nanocages.,Lucignano R, Pratesi A, Imbimbo P, Monti DM, Picone D, Messori L, Ferraro G, Merlino A Int J Mol Sci. 2022 Nov 16;23(22):14162. doi: 10.3390/ijms232214162. PMID:36430642 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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