8bel

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CIII membrane domain)

spinqualitylabelsall models 8bel, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bel is a 14 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.25Å Ligands: 3PH, CDL, FES, HEM, PC7, PGT, PTY, Q7G, UQ5, UQ7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CYB_ARATH Publication Abstract from PubMed Protein complexes of the mitochondrial respiratory chain assemble into respiratory supercomplexes. Here we present the high-resolution electron cryo-microscopy structure of the Arabidopsis respiratory supercomplex consisting of complex I and a complex III dimer, with a total of 68 protein subunits and numerous bound cofactors. A complex I-ferredoxin, subunit B14.7 and P9, a newly defined subunit of plant complex I, mediate supercomplex formation. The component complexes stabilize one another, enabling new detailed insights into their structure. We describe (1) an interrupted aqueous passage for proton translocation in the membrane arm of complex I; (2) a new coenzyme A within the carbonic anhydrase module of plant complex I defining a second catalytic centre; and (3) the water structure at the proton exit pathway of complex III(2) with a co-purified ubiquinone in the Q(O) site. We propose that the main role of the plant supercomplex is to stabilize its components in the membrane. Cryo-EM structure of the respiratory I + III(2) supercomplex from Arabidopsis thaliana at 2 A resolution.,Klusch N, Dreimann M, Senkler J, Rugen N, Kuhlbrandt W, Braun HP Nat Plants. 2023 Jan;9(1):142-156. doi: 10.1038/s41477-022-01308-6. Epub 2022 Dec , 30. PMID:36585502 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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