8bgl

Structure of the dimeric rsCherryRev1.4

spinqualitylabelsall models 8bgl, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bgl is a 2 chain structure with sequence from Discosoma sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: 1PE, ABA, EDO, PEG, Q2K, QYX Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D1MPT3_DISSP Publication Abstract from PubMed rsCherryRev1.4 has been reported as one of the reversibly photoswitchable variants of mCherry, and is an improved version with a faster off-switching speed and lower switching fatigue at high light intensities than its precursor rsCherryRev. However, rsCherryRev1.4 still has some limitations such as a tendency to dimerize as well as complex photophysical properties. Here, the crystal structure of rsCherryRev1.4 was determined at a resolution of 2 A and it was discovered that it forms a dimer that shows disulfide bonding between the protomers. Mutagenesis, gel electrophoresis and size-exclusion chromatography strongly implicate Cys24 in this process. Replacing Cys24 in rsCherryRev1.4 resulted in a much lower tendency towards dimerization, while introducing Cys24 into mCherry correspondingly increased its dimerization. In principle, this finding opens the possibility of developing redox sensors based on controlled dimerization via disulfide cross-linking in fluorescent proteins, even though the actual application of engineering such sensors still requires additional research. An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4.,Bui TYH, Dedecker P, Van Meervelt L Acta Crystallogr F Struct Biol Commun. 2023 Feb 1;79(Pt 2):38-44. doi: , 10.1107/S2053230X23000572. Epub 2023 Feb 2. PMID:36748340 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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