8bl4

Cryo-EM structure of a contractile injection system in Streptomyces coelicolor, the sheath-tube module in extended state.

spinqualitylabelsall models 8bl4, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bl4 is a 48 chain structure with sequence from Streptomyces coelicolor A3(2). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9L0N8_STRCO Publication Abstract from PubMed Contractile injection systems (CIS) are bacteriophage tail-like structures that mediate bacterial cell-cell interactions. While CIS are highly abundant across diverse bacterial phyla, representative gene clusters in Gram-positive organisms remain poorly studied. Here we characterize a CIS in the Gram-positive multicellular model organism Streptomyces coelicolor and show that, in contrast to most other CIS, S. coelicolor CIS (CIS(Sc)) mediate cell death in response to stress and impact cellular development. CIS(Sc) are expressed in the cytoplasm of vegetative hyphae and are not released into the medium. Our cryo-electron microscopy structure enabled the engineering of non-contractile and fluorescently tagged CIS(Sc) assemblies. Cryo-electron tomography showed that CIS(Sc) contraction is linked to reduced cellular integrity. Fluorescence light microscopy furthermore revealed that functional CIS(Sc) mediate cell death upon encountering different types of stress. The absence of functional CIS(Sc) had an impact on hyphal differentiation and secondary metabolite production. Finally, we identified three putative effector proteins, which when absent, phenocopied other CIS(Sc) mutants. Our results provide new functional insights into CIS in Gram-positive organisms and a framework for studying novel intracellular roles, including regulated cell death and life-cycle progression in multicellular bacteria. Cytoplasmic contractile injection systems mediate cell death in Streptomyces.,Casu B, Sallmen JW, Schlimpert S, Pilhofer M Nat Microbiol. 2023 Apr;8(4):711-726. doi: 10.1038/s41564-023-01341-x. Epub 2023 , Mar 9. PMID:36894633 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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