8bqb

CjCel5C endo-glucanase bound to CB396 covalent inhibitor

spinqualitylabelsall models 8bqb, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bqb is a 3 chain structure with sequence from Cellvibrio japonicus Ueda107. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.701Å Ligands: BGC, YLL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B3PF55_CELJU Publication Abstract from PubMed Bacteria and yeasts grow on biomass polysaccharides by expressing and excreting a complex array of glycoside hydrolase (GH) enzymes. Identification and annotation of such GH pools, which are valuable commodities for sustainable energy and chemistries, by conventional means (genomics, proteomics) are complicated, as primary sequence or secondary structure alignment with known active enzymes is not always predictive for new ones. Here we report a "low-tech", easy-to-use, and sensitive multiplexing activity-based protein-profiling platform to characterize the xyloglucan-degrading GH system excreted by the soil saprophyte, Cellvibrio japonicus, when grown on xyloglucan. A suite of activity-based probes bearing orthogonal fluorophores allows for the visualization of accessory exo-acting glycosidases, which are then identified using biotin-bearing probes. Substrate specificity of xyloglucanases is directly revealed by imbuing xyloglucan structural elements into bespoke activity-based probes. Our ABPP platform provides a highly useful tool to dissect xyloglucan-degrading systems from various sources and to rapidly select potentially useful ones. The observed specificity of the probes moreover bodes well for the study of other biomass polysaccharide-degrading systems, by modeling probe structures to those of desired substrates. A Multiplexing Activity-Based Protein-Profiling Platform for Dissection of a Native Bacterial Xyloglucan-Degrading System.,McGregor NGS, de Boer C, Foucart QPO, Beenakker T, Offen WA, Codee JDC, Willems LI, Overkleeft HS, Davies GJ ACS Cent Sci. 2023 Nov 24;9(12):2306-2314. doi: 10.1021/acscentsci.3c00831. , eCollection 2023 Dec 27. PMID:38161374 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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