8bqe | pdb_00008bqe

In situ structure of the Caulobacter crescentus S-layer

spinqualitylabelsall models 8bqe, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bqe is a 6 chain structure with sequence from Caulobacter vibrioides NA1000. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.5Å Ligands: BMA, CA, MRH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H3C8J1_CAUVN Publication Abstract from PubMed We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised likelihood target that approximates a function of the 2D experimental images. In addition, analogous to Bayesian polishing and contrast transfer function (CTF) refinement in single-particle analysis, we describe the approaches that exploit the increased signal-to-noise ratio in the averaged structure to optimise tilt-series alignments, beam-induced motions of the particles throughout the tilt-series acquisition, defoci of the individual particles, as well as higher-order optical aberrations of the microscope. Implementation of our approaches in the open-source software package RELION aims to facilitate their general use, particularly for those researchers who are already familiar with its single-particle analysis tools. We illustrate for three applications that our approaches allow structure determination from cryo-ET data to resolutions sufficient for de novo atomic modelling. A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0.,Zivanov J, Oton J, Ke Z, von Kugelgen A, Pyle E, Qu K, Morado D, Castano-Diez D, Zanetti G, Bharat TAM, Briggs JAG, Scheres SHW Elife. 2022 Dec 5;11:e83724. doi: 10.7554/eLife.83724. PMID:36468689 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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