8c2q

Silver ion-bound structure of the silver specific chaperone SilF needed for bacterial silver resistance

spinqualitylabelsall models 8c2q Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8c2q is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Ligands: AG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9ZHD1_SALTM Publication Abstract from PubMed The resistance of gram-negative bacteria to silver ions is mediated by a silver efflux pump, which mainly relies on a tripartite efflux complex SilCBA, a metallochaperone SilF and an intrinsically disordered protein SilE. However, the precise mechanism by which silver ions are extruded from the cell and the different roles of SilB, SilF, and SilE remain poorly understood. To address these questions, we employed nuclear magnetic resonance and mass spectrometry to investigate the interplay between these proteins. We first solved the solution structures of SilF in its free and Ag(+)-bound forms, and we demonstrated that SilB exhibits two silver binding sites in its N and C termini. Conversely to the homologous Cus system, we determined that SilF and SilB interact without the presence of silver ions and that the rate of silver dissociation is eight times faster when SilF is bound to SilB, indicating the formation of a SilF-Ag-SilB intermediate complex. Finally, we have shown that SilE does not bind to either SilF or SilB, regardless of the presence or absence of silver ions, further corroborating that it merely acts as a regulator that prevents the cell from being overloaded with silver. Collectively, we have provided further insights into protein interactions within the sil system that contribute to bacterial resistance to silver ions. The battle for silver binding: How the interplay between the SilE, SilF, and SilB proteins contributes to the silver efflux pump mechanism.,Arrault C, Monneau YR, Martin M, Cantrelle FX, Boll E, Chirot F, Comby Zerbino C, Walker O, Hologne M J Biol Chem. 2023 Aug;299(8):105004. doi: 10.1016/j.jbc.2023.105004. Epub 2023 , Jul 1. PMID:37394004 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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