8cmn

18mer DNA mimic Foldamer with an aliphatic linker in complex with Sac7d wild protein

spinqualitylabelsall models 8cmn, resolution 2.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8cmn is a 3 chain structure with sequence from Sulfolobus acidocaldarius DSM 639 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.65Å Ligands: GOA, V4F, V53, V5F Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DN7D_SULAC Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990] Publication Abstract from PubMed Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked. DNA Mimic Foldamer Recognition of a Chromosomal Protein.,Deepak D, Wu J, Corvaglia V, Allmendinger L, Scheckenbach M, Tinnefeld P, Huc I Angew Chem Int Ed Engl. 2024 Dec 23:e202422958. doi: 10.1002/anie.202422958. PMID:39714421 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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