8cvo

Cutibacterium acnes 30S ribosomal subunit with Sarecycline bound, head domain only in the local refined map

spinqualitylabelsall models 8cvo, resolution 2.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8cvo is a 9 chain structure with sequence from Cutibacterium acnes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.95Å Ligands: 2MG, 4OC, 5MC, G7M, MA6, MG, PSU, UR3, V7A, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS5_CUTAK With S4 and S12 plays an important role in translational accuracy.[HAMAP-Rule:MF_01307] Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.[HAMAP-Rule:MF_01307] Publication Abstract from PubMed Acne vulgaris is a chronic disfiguring skin disease affecting approximately 1 billion people worldwide, often having persistent negative effects on physical and mental health. The Gram-positive anaerobe, Cutibacterium acnes is implicated in acne pathogenesis and is, therefore, a main target for antibiotic-based acne therapy. We determined a 2.8-A resolution structure of the 70S ribosome of Cutibacterium acnes by cryogenic electron microscopy and discovered that sarecycline, a narrow-spectrum antibiotic against Cutibacterium acnes, may inhibit two active sites of this bacterium's ribosome in contrast to the one site detected previously on the model ribosome of Thermus thermophilus. Apart from the canonical binding site at the mRNA decoding center, the second binding site for sarecycline exists at the nascent peptide exit tunnel, reminiscent of the macrolides class of antibiotics. The structure also revealed Cutibacterium acnes-specific features of the ribosomal RNA and proteins. Unlike the ribosome of the Gram-negative bacterium Escherichia coli, Cutibacterium acnes ribosome has two additional proteins, bS22 and bL37, which are also present in the ribosomes of Mycobacterium smegmatis and Mycobacterium tuberculosis. We show that bS22 and bL37 have antimicrobial properties and may be involved in maintaining the healthy homeostasis of the human skin microbiome. Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism.,Lomakin IB, Devarkar SC, Patel S, Grada A, Bunick CG Nucleic Acids Res. 2023 Apr 11;51(6):2915-2930. doi: 10.1093/nar/gkad103. PMID:36864821 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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