8dyg
IL17A homodimer bound to Compound 7
Structural highlights
FunctionIL17_HUMAN Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Publication Abstract from PubMedAnti-IL17A therapies have proven effective for numerous inflammatory diseases including psoriasis, axial spondylitis and psoriatic arthritis. Modulating and/or antagonizing protein-protein interactions of IL17A cytokine binding to its cell surface receptors with oral therapies offers the promise to bring forward biologics-like efficacy in a pill to patients. We used an NMR-based fragment screen of recombinant IL17A to uncover starting points for small molecule IL17A antagonist discovery. By examining chemical shift perturbations in 2D [(1)H, (13)C-HSQC] spectra of isotopically labeled IL17A, we discovered fragments binding the cytokine at a previously undescribed site near the IL17A C-terminal region, albeit with weak affinity (> 250 microM). Importantly this binding location was distinct from previously known chemical matter modulating cytokine responses. Subsequently through analog screening, we identified related compounds that bound symmetrically in this novel site with two copies. From this observation we employed a linking strategy via structure-based drug design and obtained compounds with increased binding affinity (< 50 nM) and showed functional inhibition of IL17A-induced cellular signaling (IC(50)~1 microM). We also describe a fluorescence-based probe molecule suitable to discern/screen for additional molecules binding in this C-terminal site. Identification and structure-based drug design of cell-active inhibitors of interleukin 17A at a novel C-terminal site.,Goedken ER, Argiriadi MA, Dietrich JD, Petros AM, Krishnan N, Panchal SC, Qiu W, Wu H, Zhu H, Adams AM, Bodelle PM, Goguen L, Richardson PL, Slivka PF, Srikumaran M, Upadhyay AK, Wu B, Judge RA, Vasudevan A, Gopalakrishnan SM, Cox PB, Stoll VS, Sun C Sci Rep. 2022 Aug 26;12(1):14561. doi: 10.1038/s41598-022-18760-1. PMID:36028520[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
| ||||||||||||||||||||
